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Nike Roshe Run Hazel Color Oxford Brown,Studying mutations in bug's salivary gland could yield clues to human development A biomedical engineering major at The Johns Hopkins University has been breeding mutant fruit flies and studying their offspring to help find a gene responsible for thwarting the bug's development. Supported by an undergraduate research grant from the university, Christy A. Comeaux, 21, is working with a prominent cell biologist whose research into the way fruit fly embryos create salivary glands could shed light on the development of human organs. "Many of the genes in fruit flies are very similar to human genes," said Comeaux, a Johns Hopkins junior and a 1999 graduate of Port Neches Groves High School in Texas. Anderson Cancer Center in Houston during the summer of 2000. When she returned for her sophomore year at Johns Hopkins, she looked for a related research job and joined the lab team directed by Deborah J. Andrew, an associate professor in the Department of Cell Biology and Anatomy at the Johns Hopkins School of Medicine. "Because of my summer job, I'd had some training in genetic research techniques, but I had never worked with fruit flies," Comeaux said. "Luckily, I was surrounded by many people in the Andrew lab who were very patient and willing to answer my questions." The undergraduate soon learned how to breed flies that carry a genetic mutation. The bugs meet inside a plastic bottle. The underside of the bottle's stopper is coated with a sticky substance that collects the fertilized eggs. Comeaux learned to apply an antibody stain to these fly embryos to identify the cells that are forming the insect's salivary gland. Under a high powered microscope she was able to see how a genetic mutation can distort the shape of the developing gland and impair its ability to move into proper position within the growing embryo. Comeaux received a Provost's Undergraduate Research Award that allowed her to continue working in Andrew's lab last summer. She assisted Pamela L. Bradley, now a postdoctoral fellow, in experiments aimed at identifying and studying a mutated gene that prevented normal development of the fly embryo. "We found that a gene mutation led to the production of a protein that's shorter than it should be, so it couldn't function properly," Comeaux said. "That's why the salivary gland, and, more importantly, the fly itself didn't develop properly." The research also yielded new insights into how a developing salivary gland moves into its proper location within a fly embryo. "The work Christy carried out contributed significantly to our understanding of how cells within organs use surrounding tissues to position themselves," Andrew said. While completing her junior year courses and playing flute in the Hopkins Symphony Orchestra, Comeaux has continued to work about 20 hours a week in Andrew's lab. programs. Based on the rewarding experience in Andrew's lab, she hopes to focus on genetic research. "I never expected to be running to a research lab almost every day as an undergraduate," Comeaux said. "But it's become a major part of my education here, and it's an experience that students at other institutions may not have an opportunity to pursue." She's even learn how to peacefully co exist with the bugs that inhabit her lab. "They smell bad," Comeaux said. "But once you get used to that, you're home free." The Johns Hopkins University is recognized as the country's first graduate research university, and has been in recent years the leader among the nation's research universities in winning federal research and development grants. Since 1993, about 40 students each year have been awarded up to $2,500 to propose and conduct original research, some results of which have been published in professional journals. The awards, begun by then provost Joseph Cooper and funded through a donation from the Hodson Trust, are an important part of the university's commitment to research.

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